The Use of Indirect Immune-fluorescence Antibody Testing (IFAT) IgM And IgG In the Diagnosis of Melioidosis

Authors

  • Arumugam Janaki Department of Basic Medical Science, Hospital Sultan Ahmad Shah, Temerloh, Pahang, Malaysia
  • Nur Raziana Binti Rozi Department of Internal Medicine, Kulliyyah of Medicine, International Islamic University Malaysia (IIUM), Kuantan, Pahang, Malaysia
  • Mohammed Imad A Mustafa Mahmud Department of Basic Medical Science, Kulliyyah of Medicine, International Islamic University Malaysia (IIUM), Kuantan, Pahang, Malaysia
  • Jamalludin Bin Ab Rahman Department of Community Medicine, Kulliyyah of Medicine, International Islamic University Malaysia (IIUM), Kuantan, Pahang, Malaysia
  • Ahmad Kashfi Bin Hj Ab Rahman Department of Internal Medicine, Hospital Sultanah Nur Zahirah, Kuala Terengganu, Malaysia
  • How Soon Hin Department of Internal Medicine, Kulliyyah of Medicine, International Islamic University Malaysia (IIUM), Kuantan, Pahang, Malaysia

DOI:

https://doi.org/10.31344/ijhhs.v5i3.280

Keywords:

Melioidosis, Indirect Immunofluorescent Antibody Test, Cut-off.

Abstract

Introduction: Meliodosis is an important public health disease caused byBurkholderiapseudomallei. Early laboratory diagnosis is crucial for appropriate treatment due to its high mortality rate.

Objective: This study is conducted to assess the potential role of the in-house IFAT IgM and IgG as the serodiagnostic tool in melioidosis and to determine the cut-off levels.

Method: 40 culture-confirmed melioidosis patients were recruited. Controls consisted of a group of 40 patients without active infection and another group of 40 patients with positive blood culture for organisms other thanBurkholderiapseudomallei.

Results and Discussion: Using the receiver operating characteristic (ROC) curve, the best cut-off levels determined to diagnose melioidosis are 1:20 for IgM and 1:80 for IgG. Of these cut off levels, the sensitivity and specificity for IgM are 72.5% and 80% respectively and 65% and 87.5%respectively for IgG which also has high background seropositivity.

Conclusion: IFAT IgM at the cut-off level 1:20 is recommended for diagnosis.

International Journal of Human and Health Sciences Vol. 05 No. 03 July’21 Page: 307-314

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Published

2021-02-06

How to Cite

Janaki, A., Rozi, N. R. B., Mahmud, M. I. A. M., Rahman, J. B. A., Rahman, A. K. B. H. A., & Hin, H. S. (2021). The Use of Indirect Immune-fluorescence Antibody Testing (IFAT) IgM And IgG In the Diagnosis of Melioidosis. International Journal of Human and Health Sciences (IJHHS), 5(3), 307–314. https://doi.org/10.31344/ijhhs.v5i3.280

Issue

Vol. 5 No. 3 (2021)

Section

Original Articles

License

Copyright (c) 2021 Arumugam Janaki, Nur Raziana Binti Rozi, Mohammed Imad A Mustafa Mahmud, Jamalludin Bin Ab Rahman, Ahmad Kashfi Bin Hj Ab Rahman, How Soon Hin

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